Compound | Cross-reactivity |
---|---|
cAMP | 100% |
ADP | <0.0001% |
ATP | <0.0001% |
GTP | <0.0001% |
cGMP | <0.0001% |
GDP | <0.0001% |
AMP | <0.0001% |
GMP | 0.00817% |
Competitive ELISA Protocol:
品牌 | GenScript/金斯瑞 | 供貨周期 | 一周 |
---|---|---|---|
主要用途 | 用于尋找合適的腺苷酸環化酶激活劑或磷酸二酯酶抑制劑 | 應用領域 | 醫療衛生,食品,生物產業,制藥 |
3ˊ,5ˊ-環腺苷酸(cyclic AMP,cAMP),是細胞膜上的一種特異性腺苷酸環化酶催化細胞內的三磷酸腺苷(ATP)分解產生,是細胞內重要的第二信使,參與信號轉導過程。cAMP調節關鍵的生理過程,包括代謝,分泌,鈣穩態,肌肉收縮,細胞命運和基因轉錄等。和cAMP一樣,3ˊ,5ˊ-環鳥苷酸(cyclic GMP,cGMP),也是一種具有細胞內信息傳遞作用的第二信使。它是由鳥苷酸環化酶(GC)分解GTP產生,可被G蛋白偶聯受體(G-protein linked receptor)激活的蛋白激酶(protein kinases)活化,進而將胞外信號轉導至細胞核。環磷酸鳥苷(cGMP)可調節離子通道、糖原分解和細胞凋亡等。
抗cAMP小鼠單克隆抗體是GenScript提供的系列THE金牌抗體中的優選抗體,是業內高品質的抗體之一。該抗體及相關的試劑盒已被廣泛用于藥物篩選研究中,用于尋找合適的腺苷酸環化酶激活劑或磷酸二酯酶抑制劑。高親和力的cAMP單克隆抗體,憑借靈敏度高,特異性好,批間穩定性好,重復性好等優勢,深受眾多科學家的認可。
無非特異性反應
靈敏度高于其他公司的同類產品
提供即用型的HRP偶聯的cAMP/cGMP
凍干粉在負20度及以下,可以保存至少2年
產品編號 | 名稱 | 數量 | 價格 | 操作 |
---|---|---|---|---|
A01509 | THE™ cAMP Antibody, mAb, Mouse | ¥2500.00 | ||
M01058 | cGMP-HRP | ¥1050.00200 μl | ||
M01059 | cAMP-HRP | ¥1050.00200 μl |
1. Cross Reactivity Analysis using THE™ cAMP Antibody (Mouse)(A01509)
Compound | Cross-reactivity |
---|---|
cAMP | 100% |
ADP | <0.0001% |
ATP | <0.0001% |
GTP | <0.0001% |
cGMP | <0.0001% |
GDP | <0.0001% |
AMP | <0.0001% |
GMP | 0.00817% |
Competitive ELISA Protocol:
ELISA plate was coated with goat anti-mouse IgG antibody.
THE™ cAMP Antibody(Mouse)(GenScript, A01509) at appropriate dulition and cAMP standards or testing compounds were added into appropriate reaction wells.
After a period of incubation, cAMP-HRP conjugate (GenScript, M01059) was added and developed at room temperature.
ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature.
Stop the reaction with 1.0 N HCl and read the plate at 450 nm.